AGAR


EU SPECIFICATION

Synonyms : E 406

Definition : Agar is a hydrophilic colloidal polysaccharide consisting mainly of galactose units with a regular alternation of L and D isomeric forms. These hexoses are alternately linked with alpha-1,3 and beta-1,4 bonds in the copolymer. On about every tenth D-galactopyranose unit one of the hydroxyl groups is esterified with sulphuric acid which is neutralised by calcium, magnesium, potassium or sodium. It is extracted from certain strains of marine algae of the families Gelidiaceae and Gracilariaceae and relevant red algae of the class Rhodophyceae

Einecs : 232-658-1

Description : Agar is odourless or has a slight characteristic odour. Unground agar usually occurs in bundles consisting of thin, membranous, agglutinated strips, or in cut, flaked or granulated forms. It may be light yellowishorange, yellowish-grey to pale yellow, or colourless. It is tough when damp, brittle when dry. Powdered agar is white to yellowish-white or pale yellow. When examined in water under a microscope, agar powder appears more transparent. In chloral hydrate solution, the powdered agar appears more transparent than in water, more or less granular, striated, angular and occasionally contains frustules of diatoms. Gel strength may be standardised by the addition of dextrose and maltodextrines or sucrose

Identification : Solubility Insoluble in cold water, soluble in boiling water.


PURITY
Loss on drying Not more than 22 % (105 °C, 5 hours)
Ash Not more than 6,5 % on the anhydrous basis determined at 550 °C
Acid-insoluble ash Not more than 0.5 %
Insoluble matter Not more than 1,0 %
Starch Not detectable
Gelatin and other proteins Dissolve about 1 g of agar in 100 ml of boiling water and allow to cool of about 50 °C. To 5 ml of the solution add 5 ml of trinitrophenol solution (1 g of anhydrous trinitrophenol/100 ml of hot water). No turbidity appears within 10 minutes
Water absorption Place 5 g to agar in a 100 ml graduated cylinder, fill to the mark with water, mix and allow to stand at about 25 °C for 24 hours. Pour the contents of the cylinder through moistened glass wool, allowing the water to drain into a second 100 ml graduated cylinder. Not more than 75 ml of water is obtained
Arsenic Not more than 3 mg/kg
Lead Not more than 5 mg/kg
Mercury Not more than 1 mg/kg
Cadmium Not more than 1 mg/kg
Microbiological Criteria
Total plate count Not more than 5 000 colonies per gram
Yeast and moulds Not more than 300 colonies per gram
Escherichia coli Absent in 5 g
Salmonella spp. Absent in 5 g

PACKING & STORAGE

Packing 25 Kg, Paper Bags with inner PE liner Bags
Shelf Life 2 Years After Manufacturing Date, Under Recommended Conditions
Storage Cold And Dry Conditions, Sealed Packaging